Andrewes, P.; Gill, B.D.; Holroyd, S.E.; Johnson, R.; Lewis, A.; MacKenzie, A.; Vyssotski, M. (2022). In Understanding and improving the functional and nutritional properties of milk. T. Huppertz & T. Vasiljevic (eds.) Burleigh Dodds Science Publishing: Cambridge, UK
Understanding and improving the functional and nutritional properties of milk reviews the latest research on the remarkable range of functional and nutritional properties of milk that make it both a key food source and ingredient in a wide range of dairy products. The collection discusses proteins, lipids, carbohydrates and other components of milk, as well as how our understanding can be used to optimise the quality of milk and dairy products such as cheese and yoghurt.
Metabolite profiling involves the measurement of low-molecular-weight metabolites and their intermediates that originate within biological organisms. Many metabolomics studies have focused on identifying disease biomarkers in humans and other animals, identifying breeding and quality markers for plants and animals in food production, and investigating new pharmaceutical drug candidates.
This chapter introduces mass spectrometry instrumentation and experiments that are used to carry out metabolite profiling. The application of different mass spectrometry techniques to various sample types is discussed, as well as the application of different instrumentation to untargeted experiments for acquiring metabolic “fingerprint” profiles of biological organisms and targeted experiments used for analyte quantification. The aspects of sample preparation are discussed along with the workflows for data processing. Different data analytics tools describe how to establish which metabolic pathways are enriched or depleted by determining which metabolites are up- or downregulated using volcano plots and heat maps.
show abstract
Understanding and improving the functional and nutritional properties of milk reviews the latest research on the remarkable range of functional and nutritional properties of milk that make it both a key food source and ingredient in a wide range of dairy products. The collection discusses proteins, lipids, carbohydrates and other components of milk, as well as how our understanding can be used to optimise the quality of milk and dairy products such as cheese and yoghurt.
Published: 15 March 2022
Book Citation Chapter Manuscriptshow abstract
Metabolite profiling involves the measurement of low-molecular-weight metabolites and their intermediates that originate within biological organisms. Many metabolomics studies have focused on identifying disease biomarkers in humans and other animals, identifying breeding and quality markers for plants and animals in food production, and investigating new pharmaceutical drug candidates.
This chapter introduces mass spectrometry instrumentation and experiments that are used to carry out metabolite profiling. The application of different mass spectrometry techniques to various sample types is discussed, as well as the application of different instrumentation to untargeted experiments for acquiring metabolic “fingerprint” profiles of biological organisms and targeted experiments used for analyte quantification. The aspects of sample preparation are discussed along with the workflows for data processing. Different data analytics tools describe how to establish which metabolic pathways are enriched or depleted by determining which metabolites are up- or downregulated using volcano plots and heat maps.
Published: 30 September 2025
Book Citation Chapter Manuscript