ISO Standard 20636:2018; Infant Formula—Determination of Vitamin D by Liquid Chromatography-Mass Spectrometry

Samples are saponified at high temperature then lipid soluble components are extracted into isooctane. A portion of the isooctane layer is transferred, washed, and an aliquot of 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) is added to derivatise vitamin D to form a high molecular mass, easily ionisable adduct. The vitamin D-adduct is then re extracted into a small volume of acetonitrile and analysed by reversed-phase liquid chromatography. Detection is by mass spectrometry using multiple reaction monitoring (MRM). Stable isotope labelled d6-vitamin D2 and d6-vitamin D3 internal standards are used for quantitation to correct for losses in extraction and any variation in derivatisation and ionisation efficiencies.

Method Link
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

International Standards Organisation (2018) Infant formula—Determination of vitamin D by liquid chromatography-mass spectrometry: Geneva, Switzerland. Standard 20636:2018

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