The β‑Lactoglobulin Content of Bovine Milk: Development and Spplication of a Biosensor Immunoassay

Harvey E. Indyk, Selena Hart, Theo Meerkerk, Brendon D. Gill, David C. Woollard

An optical biosensor immunoassay exploiting surface plasmon resonance is described for the quantification of β‑lactoglobulin in milk. Samples were diluted with buffer, and the protein estimated from binding with a polyclonal antibody immobilised on the sensor surface. Analytical method performance characteristics including range, detection limit, precision and accuracy were determined and reported. The temporal variability in the β‑lactoglobulin content of milk from pasture-fed cows during early lactation and across a production season was investigated. The content of β‑lactoglobulin decreased from >10 mg mL‑1 in early colostrum to <5 mg mL‑1 in mature milk, and the β‑lactoglobulin content of skim milk powder trended from 25 to 60 mg g‑1 across a season. In view of its allergenicity, these data will improve understanding of the expression of innate β‑lactoglobulin in the milk of pasture-grazed dairy herds, thereby providing information that is applicable to the formulation of bovine milk-based products.

Gill et al. (2016) Int. Dairy J., 73, 68–73.Journal Link
Indyk et al. (2017) Int. Dairy J., 73, 68–73.Article PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Indyk, H.E., Hart, S., Meerkerk, T., Gill, B.D., & Woollard, D.C. (2017) Int.  Dairy J. 73, 68‑73
doi: 10.1016/j.idairyj.2017.05.010

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