Brendon Gill PhD MSc(hons) GradDipT FAOACI


The determination of vitamin D3 and 25-hydroxyvitamin D3 in early lactation and seasonal bovine milk

Brendon D. Gill, Xiangjun Zhu, Harvey E. Indyk

There is a need to account for the content of 25‑hydroxyvitamin D3 (25OH‑D3) in foods to more accurately estimate dietary vitamin D intake, given its higher biological activity. A high-performance liquid chromatography-tandem mass spectrometry method was applied to the determination of vitamin D3 and 25OH‑D3 in bovine milk obtained during early lactation and over the course of a full milking season. In this seasonal study of bovine milk, vitamin D3 levels ranged from 167 ng L‑1 in winter to 615 ng L‑1 in summer, whereas the content of 25OH‑D3 in bovine milk was <50 ng L‑1 and showed little variation. This study will provide manufacturers with data concerning endogenous vitamin D content that will enhance formulation capability related to the production of bovine milk-based paediatric products.


Gill et al. (2016) Int. Dairy J., 63, 29–34.Journal Link
Gill et al. (2016) Int. Dairy J., 63, 29–34.Article PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Gill, B.D., Zhu, X., & Indyk, H.E. (2016) Int. Dairy J. 63, 29‑34
doi: 10.1016/j.idairyj.2016.07.010


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