ISO Standard 20638:2015; Infant formula—Determination of Nucleotides by Liquid Chromatography

Sample is dissolved in high-salt solution to inhibit protein and fat interactions. The 5′‑mononucleotides—uridine 5′‑monophosphate (UMP), inosine 5′‑monophosphate (IMP), adenosine 5′‑monophosphate (AMP), guanosine 5′‑monophosphate (GMP), and cytidine 5′‑monophosphate (CMP) are separated from the sample matrix by strong-anion exchange solid-phase extraction (SPE), followed by chromatographic analysis using a C18 stationary phase with gradient elution, UV detection, and quantitation by an internal standard (IS) technique using thymidine 5′‑monophosphate (TMP).

Method Link
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

International Standards Organisation (2015) Infant formula—Determination of Nucleotides by Liquid Chromatography: Geneva, Switzerland. Standard 20638:2015

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