Brendon Gill PhD MSc(hons) GradDipT FAOACI


Analysis of Nucleotide 5′‑Monophosphates in Infant Formulas by HPLC-UV: Collaborative Study, Final Action 2011.20

Brendon D. Gill and Harvey E. Indyk

A collaborative study was conducted on AOAC First Action Method 2011.20: 5′‑Mononucleotides in Infant Formula and Adult/Pediatric Nutritional Formula. After the successful analysis of National Institute of Standards and Technology (NIST) 1849a Standard Reference Material (SRM) as a practice sample, 12 laboratories participated in the analysis of duplicate samples of six different infant formula products. The samples were dissolved in high-salt solution to inhibit protein and fat interactions, with the nucleotides [uridine 5′‑monophosphate (UMP), inosine 5′‑monophosphate (IMP), adenosine 5′‑monophosphate (AMP), guanosine 5′‑monophosphate (GMP), and cytidine 5′‑monophosphate (CMP)] separated from the sample matrix by strong-anion exchange SPE, followed by chromatographic analysis using a C18 stationary phase with gradient elution, UV detection, and quantitation by an internal standard technique using thymidine 5′‑monophosphate. For nucleotidesupplemented products, precision is within the Standard Method Performance Requirements (SMPR) 2011.008 target reproducibility limit of <11%, with the reproducibility RSD (RSDR) estimated at 7.1–8.7% for CMP, 7.9–9.0% for UMP, 2.8–7.7% for GMP, 5.5–10.3% for IMP, and 2.7–6.2% for AMP, and Horwitz ratio (HorRat) values of 0.9–1.0 for CMP, 0.9–1.0 for UMP, 0.3–0.7 for GMP, 0.6–1.0 for IMP, and 0.3–0.7 for AMP.


MLT Collaborators
Sneh Bhandari, Silliker Laboratories, United States of America
Greg Jaudzems, Nestlé, United States of America
George Joseph, AsureQuality, New Zealand
Sabine Meng Jensen, Eurofins, Denmark
Sam Murray and Paul McNabb, Cawthron Institute, New Zealand
Sheila Saldo and Holly Huo, Fonterra Co-operative Group, New Zealand
Steve Tennyson and Scott Christiansen, Perrigo Nutritionals, United States of America
Erika Vacha and John Austad, Covance, United States of America
Martijn Vermeulen, TNO Triskelion, The Netherlands
Sarita Vyas and Pat Vyas, Eurofins, China
Norman White, Abbott Laboratories, United States of America
Bolong Wu, CAIQ, China


Gill and Indyk (2015) J. AOAC Int. 98, 971-979.Journal Link
Gill and Indyk (2015) J. AOAC Int. 98, 971-979.Article PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Gill, B.D., & Indyk, H.E. (2015) J. AOAC Int. 98, 971‑979
doi: 10.5740/jaoacint.15 050


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