Brendon Gill PhD MSc(hons) GradDipT FAOACI


Application of an LC-UV method to estimate lutein recovery during infant formula manufacture.

Harvey E. Indyk, Brendon D. Gill, Jane M. Broughton, and David C. Woollard

Lutein is a carotenoid that is considered to be important to the integrity of the retina and is therefore increasingly being supplemented into bovine milk-based paediatric formulae to levels equivalent to those found in human milk. A simple analytical method has been developed and intra-laboratory validated to facilitate routine in-process control of lutein addition. The method involves dilution of a carotenoid premix, followed by C30 reversed-phase liquid chromatographic separation of lutein, zeaxanthin and β‑carotene, with detection and quantitation at 450 nm. The method performance parameters include range (0–700 ng mL-1), method limit of detection (0.08 μg g-1), recovery (95.5–109.5%) and precision (1.2% RSDr). The method has been applied to an evaluation of lutein recovery (95.6–104.2%) through the manufacture of paediatric formulae, which confirms that lutein loss through the entire process is insignificant.


Indyk et al. (2014) Int Dairy J. 38, 82-86.Journal Link
Indyk et al. (2014) Int Dairy J. 38, 82-86.Article PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Indyk, H.E., Gill, B.D., Broughton, J.M., & Woollard, D.C. (2014) Int. Dairy J. 38, 82‑86
doi: 10.1016/j.idairyj.2014.02.010


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