Brendon Gill PhD MSc(hons) GradDipT FAOACI


Inside Laboratory Management

First Action Official Method 2011.20 (5′‑Mononucleotides in Infant Formula and Nutritional Formula) Successfully Completes SLV; Method to Proceed to Multilaboratory Study

An SLV of AOAC Method 2011.20 was performed with the assessment of method recvoery, linearity, precision and LOD and LOQ to demonstrate compliance with the SPIFAN SMPR utilizing a SPIFAN test materials kit. The method met all the criteria specified in the SMPR at th 0.1, 1, and 5 mg/dL concentration levels. Although the method did not meet all the crieria for precision and accuracy at the lowest concetration (0.02 mg/dL) in the SMPR, this conctration is well below levels measured in nucleitde-supplemented infant formulas. Further evaluation of the method, including bias, intermediate precision, and selectivity was also performed. The method was found to be suitable for its intended purpose of measuring 5′‑mononucleotides in infant formula.


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Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Gill, B.D., Indyk, H.E., & Hughes, D. (2011) Inside Lab. Manag. Sept/Oct 2013, 43‑45


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