Brendon Gill PhD MSc(hons) GradDipT FAOACI


Analysis of nucleosides and nucleotides in infant formula by liquid chromatography-tandem mass spectrometry.

Brendon D. Gill, Harvey E. Indyk, and Merilyn Manley-Harris

A method for the simultaneous analysis of nucleosides and nucleotides in infant formula using reversed phase liquid chromatography-tandem mass spectrometry is described. This approach is advantageous for compliance testing of infant formula over other LC-MS methods in which only nucleotides or nucleosides are measured. Following sample dissolution, protein was removed by centrifugal ultrafiltration. Chromatographic analyses were performed using a C18 stationary phase and gradient elution of an ammonium acetate/bicarbonate buffer, mass spectrometric detection and quantitation by a stable isotope labeled internal standard technique. A single laboratory validation was performed, with spike recoveries of 80.1–112.9% and repeatability relative standard deviations of 1.9–7.2%. Accuracy as bias was demonstrated against reference values for NIST 1849a certified reference material. The method has been validated for the analysis of bovine milk-based, soy-based, caprine milk-based and hydrolysed milk protein-based infant formulae.


Gill et al. (2013) Anal. Bioanal. Chem. 405, 5311-5319.Journal Link
Gill et al. (2013) Anal. Bioanal. Chem. 405, 5311-5319.Article PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Gill, B.D., Indyk, H.E., & Manley-Harris, M. (2013) Anal. Bioanal. Chem. 405, 5311‑5319
doi: 10.1007/s00216-013-6935-9


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