Brendon Gill PhD MSc(hons) GradDipT FAOACI


AOAC Official Method 2011.20

Analysis of 5′‑Mononucleotides in Infant Formula and Adult/Pediatric Nutritional Formula

Sample is dissolved in high-salt solution to inhibit protein and fat interactions. The 5′‑mononucleotides—uridine 5′‑monophosphate (UMP), inosine 5′‑monophosphate (IMP), adenosine 5′‑monophosphate (AMP), guanosine 5′‑monophosphate (GMP), and cytidine 5′‑monophosphate (CMP) are separated from the sample matrix by strong-anion exchange solid-phase extraction (SPE), followed by chromatographic analysis using a C18 stationary phase with gradient elution, UV detection, and quantitation by an internal standard (IS) technique using thymidine 5′‑monophosphate (TMP).


Method Link
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

AOAC International (2016) 5′‑Mononucleotides in Infant Formula and Adult/Pediatric Nutritional Formula: Gaithersburg MD, USA. Official Method 2011.20

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