Brendon Gill PhD MSc(hons) GradDipT FAOACI


A Liquid Chromatographic Method for Routine Analysis of 5′‑Mononucleotides in Pediatric Formulas

Brendon D. Gill, Harvey E. Indyk, Maureen C. Kumar, Nathan K. Sievwright, and Merilyn Manley-Harris

An RP‑HPLC method for the routine determination of supplemented 5′ mononucleotides (uridine 5′ monophosphate, inosine 5′ monophosphate, adenosine 5′ monophosphate, guanosine 5′ monophosphate, and cytidine 5′ monophosphate) in pediatric formulas and milk products is described. Following sample dissolution, potential interferences were removed by anion-exchange SPE. Chromatographic analyses were performed using a C18 stationary phase with gradient elution, UV detection, and quantitation by an internal standard technique. A single-laboratory validation was performed, with recoveries of 92–101% and repeatability RSDs of 1.0–2.3%. The method was optimized for the rapid, routine analysis of nucleotide-supplemented bovine milk-based infant and follow-on formulas.


Gill et al. (2010) J. AOAC INt., 93, 966-973.Journal Link
Gill et al. (2010) J. AOAC Int., 93, 966-973.Article PDF
Poster PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Gill, B.D., Indyk, H.E., Kumar, M.C., Sievwright, N.K., & Manley-Harris, M. (2010) J. AOAC Int. 93, 966‑973

Poster presented at the 124th Annual AOAC International Conference, Orlando FL, USA; September 2010.


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