Brendon Gill PhD MSc(hons) GradDipT FAOACI


Liquid chromatographic method for the determination of lutein in milk and pediatric formulas

Brendon D. Gill and Harvey E. Indyk

A simple and rapid method has been developed for routine compliance testing of lutein in pediatric formulas. Following a mild saponification procedure (70 °C for 10 min) demonstrated to be benign to lutein, lipophilic components were partitioned by a single extraction into hexane:diisopropyl ether (75:25 v/v) and concentrated in mobile phase. Baseline chromatographic separation of lutein and zeaxanthin (internal standard) was achieved using a C30 column and isocratic elution with methanol: dichloromethane (70:30 v/v). Lutein concentration was quantitated against zeaxanthin, which corrected for incomplete extraction recovery. Performance parameters assessed included recovery (101–108%) and repeatability (2.2% RSD). The method was applied to the analysis of lutein supplemented pediatric formulas, unsupplemented milk powders, and bovine and human milk.


Gill & Indyk (2008) Int. Dairy J., 18, 894-898.Journal Link
Gill & Indyk (2008) Int. Dairy J., 18, 894-898.Article PDF
Poster PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Gill, B.D., & Indyk, H.E. (2008) Int. Dairy J. 18, 894‑898
doi: 10.1016/j.idairyj.2008.02.004

Poster presented at the 122nd Annual AOAC International Conference, Dallas TX, USA; September 2008.


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