Brendon Gill PhD MSc(hons) GradDipT FAOACI


Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

Brendon D. Gill and Harvey E. Indyk

A method for the simultaneous determination in milk of the 5′‑mononucleotides adenosine 5′‑monophosphate, cytidine 5′‑monophosphate, guanosine 5′‑monophosphate, inosine 5′‑monophosphate and uridine 5′‑monophosphate and their corresponding nucleosides is described. Following deproteinisation, the sample extract was analysed by reversed-phase liquid chromatography, whereby chromatographic separation was achieved using a polymer grafted silica C18 column, gradient elution with a simple binary mobile phase and UV detection by photodiode array. Performance parameters included recoveries of 95.5–105.2% and precision evaluated as 3.42–6.38% relative standard deviation. The described technique has been applied to the analysis of bovine and human milk, a range of commercial bovine milk-based infant and follow-on formulas, a seasonal study of skim milk powders and an assessment of alkaline phosphatase influence on nucleotide retention.


Gill & Indyk (2007) Int. Dairy. J. 17, 596-605.Journal Link
Gill & Indyk (2007) Int. Dairy. J. 17, 596-605.Article PDF
Poster PDF
Reference Style

Journal: Journal of AOAC International
Publisher: AOAC International

Gill, B.D., & Indyk, H.E. (2007) Int. Dairy J. 17, 596‑605
doi: 10.1016/j.idairyj.2006.08.001

Poster presented at the 120th Annual AOAC International Conference, Minneapolis MN, USA; September 2006 (co-author Harvey Indyk presented).


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